Does anybody know or have a software for designing a network between microRNAs and their targets?

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How can a convenient result about miRNA be obtained by RT-PCR from FFPE breast tissues?

Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...

31 December 2019 3,938 3 View

Can somebody interpret the following images belonged to a miRNA pretest?

Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...

31 December 2019 7,334 2 View

Can I synthesize single strand cDNA for miRNA from total RNA? or Can I use Total RNA extracted to isolate microRNA?

Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...

31 December 2019 4,525 5 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

31 December 2019 7,271 2 View

Can I use DNase I during washing microRNA Enrichment Column?

Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...

31 December 2019 9,178 4 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

06 July 2019 852 4 View

Can we put formalin fixed breast tissue in 1x PBS for less than 20h? or Can I put formalin fixed breast tissue in liquid nitrogen after 10hrs?

Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....

06 July 2019 6,271 4 View

Could somebody please recommend me which microRNAs or genes associated with breast cancer are good to find new result?

I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...

04 May 2019 2,991 4 View

Who can help me to learn tissue culture and cell lines?

Dear Sir/ Madam I am phD student. I do not know the process of tissue culture for breast Cancer. I want to do but I am not familiar with it. I have a tissue culture room but I do not know how to...

03 April 2019 2,205 6 View

Does anyone know or have any templet or information structure to see the structure of protein?

I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website...

05 June 2016 8,462 0 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

What researches are there for satisfaction level of the hospital attachment for student nurses ?

I am doing a study on nursing students satisfaction on the hospital attachment whether they are satisfied with the clinical attachment or not. I need more research studies on this

03 August 2024 6,985 2 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

For a case-control study for Breast Cancer incidents in a particular County in USA, what data can be used as Control? E.g. colorectal cancer?

The County health department is providing Breast cancer incident data. If colorectal cancer cases could be used as control, then we can get respective data from health department as well.

22 July 2024 9,954 4 View

About Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images ?

Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images

21 July 2024 5,471 0 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Contaminant cells in pancreatic cancer patient derived organoids?

Hello everyone, I am cultivating patient derived organoids from pancreatic cancer patients and I saw at the bottom of my domes some "strange" cells other than my usual organoids. I know I should...

15 July 2024 762 3 View

Oncologist at Visakhapatnam interested to collaborate?

Calling all medical professionals and researchers! I'm seeking recommendations for an oncologist in Visakhapatnam who can collaborate on my PhD thesis. My research focuses on stage 1 and 2 gastric...

14 July 2024 936 0 View