How can a convenient result about miRNA be obtained by RT-PCR from FFPE breast tissues?

Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...

31 December 2019 3,847 3 View

Can somebody interpret the following images belonged to a miRNA pretest?

Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...

31 December 2019 7,250 2 View

Can I synthesize single strand cDNA for miRNA from total RNA? or Can I use Total RNA extracted to isolate microRNA?

Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...

31 December 2019 4,430 5 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

31 December 2019 7,189 2 View

Can I use DNase I during washing microRNA Enrichment Column?

Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...

31 December 2019 9,049 4 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

06 July 2019 731 4 View

Can we put formalin fixed breast tissue in 1x PBS for less than 20h? or Can I put formalin fixed breast tissue in liquid nitrogen after 10hrs?

Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....

06 July 2019 6,124 4 View

Does anybody know or have a software for designing a network between microRNAs and their targets?

I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets

04 May 2019 4,074 2 View

Could somebody please recommend me which microRNAs or genes associated with breast cancer are good to find new result?

I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...

04 May 2019 2,913 4 View

Does anyone know or have any templet or information structure to see the structure of protein?

I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website...

05 June 2016 8,383 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View