Can I use DNase I during washing microRNA Enrichment Column?

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How can a convenient result about miRNA be obtained by RT-PCR from FFPE breast tissues?

Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...

31 December 2019 3,847 3 View

Can somebody interpret the following images belonged to a miRNA pretest?

Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...

31 December 2019 7,250 2 View

Can I synthesize single strand cDNA for miRNA from total RNA? or Can I use Total RNA extracted to isolate microRNA?

Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...

31 December 2019 4,430 5 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

31 December 2019 7,189 2 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

06 July 2019 731 4 View

Can we put formalin fixed breast tissue in 1x PBS for less than 20h? or Can I put formalin fixed breast tissue in liquid nitrogen after 10hrs?

Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....

06 July 2019 6,124 4 View

Does anybody know or have a software for designing a network between microRNAs and their targets?

I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets

04 May 2019 4,074 2 View

Could somebody please recommend me which microRNAs or genes associated with breast cancer are good to find new result?

I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...

04 May 2019 2,913 4 View

Who can help me to learn tissue culture and cell lines?

Dear Sir/ Madam I am phD student. I do not know the process of tissue culture for breast Cancer. I want to do but I am not familiar with it. I have a tissue culture room but I do not know how to...

03 April 2019 2,126 6 View

Does anyone know or have any templet or information structure to see the structure of protein?

I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website...

05 June 2016 8,383 0 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

How is linear form of plasmid circularized?

Hi all, I'm working on transforming E. coli with plasmid in a mixture of linear and circular form. What happened to the linear form once uptake? Do cells ligate them directly? Or cell DNase...

13 January 2021 8,336 4 View