Sevan Majed

11 Questions 13 Answers 0 Followers

Questions related from Sevan Majed

How can a convenient result about miRNA be obtained by RT-PCR from FFPE breast tissues?

Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...

01 January 2020 3,913 3 View

Can somebody interpret the following images belonged to a miRNA pretest?

Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...

01 January 2020 7,320 2 View

Can I synthesize single strand cDNA for miRNA from total RNA? or Can I use Total RNA extracted to isolate microRNA?

Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...

01 January 2020 4,502 5 View

Does anybody has the protocol of MicroRNA purification from FFPE block tissue?

Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.

01 January 2020 7,253 2 View

Can I use DNase I during washing microRNA Enrichment Column?

Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...

01 January 2020 9,154 4 View

Can we put formalin fixed breast tissue in 1x PBS for less than 20h? or Can I put formalin fixed breast tissue in liquid nitrogen after 10hrs?

Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....

07 July 2019 6,246 4 View

Whether 1XPBS or 10X PBS is better for washing the breast cancer sample that placed in formalin? how many times can I wash the sample with the PBS?

Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...

07 July 2019 815 4 View

Could somebody please recommend me which microRNAs or genes associated with breast cancer are good to find new result?

I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...

05 May 2019 2,973 4 View

Does anybody know or have a software for designing a network between microRNAs and their targets?

I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets

05 May 2019 4,146 2 View

Who can help me to learn tissue culture and cell lines?

Dear Sir/ Madam I am phD student. I do not know the process of tissue culture for breast Cancer. I want to do but I am not familiar with it. I have a tissue culture room but I do not know how to...

04 April 2019 2,184 6 View

Does anyone know or have any templet or information structure to see the structure of protein?

I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website...

06 June 2016 8,445 0 View