I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website to see the structure of it.
Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...
31 December 2019 3,847 3 View
Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...
31 December 2019 7,250 2 View
Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...
31 December 2019 4,430 5 View
Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.
31 December 2019 7,189 2 View
Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...
31 December 2019 9,049 4 View
Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...
06 July 2019 731 4 View
Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....
06 July 2019 6,124 4 View
I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets
04 May 2019 4,074 2 View
I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...
04 May 2019 2,913 4 View
Dear Sir/ Madam I am phD student. I do not know the process of tissue culture for breast Cancer. I want to do but I am not familiar with it. I have a tissue culture room but I do not know how to...
03 April 2019 2,126 6 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...
03 March 2021 961 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...
02 March 2021 728 2 View
I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.
02 March 2021 7,735 3 View
For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...
01 March 2021 5,035 2 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View