I have only a protein sequence that has not any information structure in the pdb website. I have also no idea how to build information structure. so, I need somebody to give and show me a website to see the structure of it.
Dears/ Madams I am a PhD student My research works are about some miRNAs from FFPE block breast tissues. I have use Norgene kits for miRNAs purification and abm kits primers and universal primers...
31 December 2019 3,938 3 View
Dear/Madam I am a Ph.D. student. I am working on microRNA expression in FFPE breast tissue. Today I did a pretest for one sample (Cancer) to detect one microRNA. I ran a breast cancer sample and...
31 December 2019 7,334 2 View
Dear/ Madam I am a little confused about extraction of microRNA from FFE block. whether total RNA is first extracted from FFE tissue and then miRNA is extracted from total RNA or Can I...
31 December 2019 4,525 5 View
Dear Madam I need a good protocol for MicroRNA purification from FFPE-breast tissue. please If anybody has it, can you send it to me.
31 December 2019 7,271 2 View
Dear/Madam I want to extract microRNA from FFPE tissue. I have used Norgen Kits for miRNA purification but my result is not good when I run Real Time PCR. I donot know why? . I think I have had...
31 December 2019 9,178 4 View
Dear/Madam I am ph.D student. pathologists give me the breast cancer samples that placed in formalin for 5 days. I have just 1X PBS and 10X PBS. I just want to know, which one is better to wash...
06 July 2019 852 4 View
Dear/ Madame I have a problem with sample collection. In may city hospital, the surgeons put directly the tissue cancers in formalin after operation but you know, formalin damages RNA quality....
06 July 2019 6,271 4 View
I am a ph.D student. I am going to work on some microRNAs related to breast cancer. I would like to design an interaction between selected the microRNAs and their targets
04 May 2019 4,168 2 View
I am going to work some microRNAs regulated some gene products. I want somebody to give some microRNAs linked to breast caancer genes. Does anybody tell some websites/ tools to analyse them? Best...
04 May 2019 2,991 4 View
Dear Sir/ Madam I am phD student. I do not know the process of tissue culture for breast Cancer. I want to do but I am not familiar with it. I have a tissue culture room but I do not know how to...
03 April 2019 2,205 6 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Is this website real? https://isar.org.in/event/registration.php?id=2434532
08 August 2024 484 1 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...
07 August 2024 2,598 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Dear friends, does anybody know that is it legal to upload a graphical abstract previously published on your paper's first page to a website such as figshare.com under CC-BY-4.0 license? Thanks.
05 August 2024 7,098 3 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View