At which step of a staining for FACS do I add Hoechst? Can I use it like DAPI?

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How do you collect the celiac ganglion from mice?

Does anyone know how to / anatomical landmarks to collect the celiac ganglion?

06 December 2023 8,709 0 View

Can you tell me a protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

Hello everyone, Can you tell me a standardized protocol for the differentiation of mesenchymal stem cells into oligodendrocytes?

14 November 2023 1,546 1 View

Freeman Tukey test: is there an online tool?

Hi, I have data in form of counts (number) and I wanted to run a statistical test to compare the counts between groups to see if there is statistically significant difference between groups. I...

24 October 2023 1,545 3 View

How can I prevent exosomes from clumping together in PBS for NTA after isolation?

I used the ExoSpin kit to isolate EVs from Plasma using 0.22 double filtration and 10KDa Ultrafiltration device and resuspended in PBS (cacl and mg free) and still find that particles are clumping...

13 August 2023 4,773 0 View

Has anyone had problems with the PyroMark Q48 cartridges?

Hi everyone, a few months ago we bought the PyroMark Q48 to replace the old Q96, but we used it very little due to continuous malfunctions of the cartridge with enzyme and substrate. We've already...

23 July 2023 8,854 0 View

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices?

What is the optimal concentration of glutamate for patch clamp recordings in mouse brain slices? Can someone provide a study on the dose-response relationship of glutamate in brain slice preparations?

11 July 2023 9,547 1 View

Is it possible to quantify 16S regions in a sample of WGS data?

Hi, I would like to know if there is any software readily available which allows the identification and quantification for 16S regions in WGS data. Thanks n advance for any advice/help :)

15 May 2023 1,177 0 View

How do you estimate parts of the biofilm being sediments or dead organic material of biomass collected from river stones?

We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier,...

27 March 2023 2,413 1 View

I keep encountering this error when trying the Cast transects in DSAS?

--------------------------- DSAS Error --------------------------- An unexpected error occurred. Please refer to the log file (C:\Users\Deighl\AppData\Roaming\Corona Environmental Consulting\DSAS...

23 January 2023 7,611 0 View

What happens when legally mandated Patient nurse ratios are exceeded? Due to local emergencies etc?

I'm currently writing my dissertation (3rd year adult nursing student) on the impact of patient nurse ratio in the UK, and have been researching around Australia and California where there are...

17 December 2022 8,316 6 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View