We measured chlorophyll, dry mass and ash-free dry mass and after we calculated autotrophic index (AI). I found in Hauer, F. R., & G. A. Lamberti, 2017. Methods in stream ecology. Elsevier, San Diego, California: “Hotchkiss and Hall (2015), for example, estimated that only 7-20% of the biofilm biomass they studied was metabolically active.”