Do you have any suggestions for neurosphere assay protocol, using adult mice?

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Does anyone have good protocol for dissociation of hippocampal neurons from adult mouse (flow cytometry/RNA seq)?

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Are there any reliable antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus?

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Any (hippocampal) culture and cell line useful to investigate epigenetic regulation (ie. high proliferation rate)?

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Problems in using mouse host primary antibodies on mouse brain sample (IF/free floating staining).

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Having difficulty IF staining on 5XFAD mouse brain sample (free floating).

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Are there any rodent behavioural experiments that can substitute contextual fear conditioning test?

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Reversal learning deficit, but normal memory extinction on Morris water maze test

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Why activated CAR-Jurkat cell could not kill targets?

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Why I can't see any band in SDS-PAGE?

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Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

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How to isolate lymphocytes from mouse spleen?

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What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

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What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

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EDTA buffer solution?

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David S. Thaler

PROTOCOL REVIEW

Eyes Wide Open: A Critical Review of Sphere-Formation as an Assay for Stem Cells

Erika Pastrana, Violeta Silva-Vargas, Fiona Doetsch📷

Nature Publishing Group

Cell Stem Cell Volume 8, Issue 5, p486–498, 6 May 2011

Open ArchivePlumX Metrics📷DOI: https://doi.org/10.1016/j.stem.2011.04.007

Cited ~500 times

Yuka Obayashi

Hello David, I am very sorry for delayed reply, but thank you very much for your suggestion! It was helpful!