Yuka Obayashi

8 Questions 17 Answers 0 Followers

Questions related from Yuka Obayashi

Does anyone have good protocol for dissociation of hippocampal neurons from adult mouse (flow cytometry/RNA seq)?

I am looking for any good protocol for dissociation of single hippocampal neurons from adult mice. We'll perform flow cytometry and eventually work on qPCR. As our lab is not specialized for...

07 July 2019 8,382 2 View

Are there any reliable antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus?

I have Camk2a-Cre mice model, and would like to have antibodies for excitatory (glutamatergic) and inhibitory (GABAnergic) neurons in hippocampus CA1 regions. Do you know any good antibodies for...

11 November 2018 9,529 4 View

Do you have any suggestions for neurosphere assay protocol, using adult mice?

Do you have any suggestions for growth factors (EGF and FGF) effective/useful for proliferation medium? How do you sterilize surgical equipments - autoclave or buffer? Any tips to share -...

02 February 2018 1,180 2 View

Any (hippocampal) culture and cell line useful to investigate epigenetic regulation (ie. high proliferation rate)?

I am currently looking for (preferably hippocampus) culture/neurosphere/cell line with high proliferation rate, which are available to purchase in Canada.

02 February 2018 4,773 2 View

Problems in using mouse host primary antibodies on mouse brain sample (IF/free floating staining).

I am doing free floating immunofluorescent staining on my mouse brain sample. I've been having difficulty using rodent host (ie. mouse, rat, guinea pig) primary antibodies, and I don't know why....

01 January 1970 6,992 13 View

Having difficulty IF staining on 5XFAD mouse brain sample (free floating).

I am not sure if we are supposed to change staining protocol for different mouse strains, but I am having difficulty staining (free floating immunofluorescent staining) on my 5XFAD mouse brain...

01 January 1970 3,298 5 View

Are there any rodent behavioural experiments that can substitute contextual fear conditioning test?

I am investigating the role of histone modification during hippocampal memory formation. It is the best if I could perform contextual fear conditioning on mice. However, we unfortunately do not...

01 January 1970 9,584 1 View

Reversal learning deficit, but normal memory extinction on Morris water maze test

I am wondering if anyone has seen the results of morris water maze like this; - normal acquisition (decrease in escape latency) BUT - significant deficit in reversal learning - normal memory...

01 January 1970 8,092 4 View