Does anyone have good protocol for dissociation of hippocampal neurons from adult mouse (flow cytometry/RNA seq)?

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Bahman Sadeghi

Please check the following paper in which investigated the effect of CA1 and CA3 pyramidal neuron dissociation on brain transcriptomes:

Article Hippocampal transcriptomic responses to enzyme‐mediated cell...

But if you are looking for a full protocol from a to z, that includes necessary reagents, materials as well as how to harvest adult mice brains, hippocampus dissection, homogenization, myelin removal,..., to FACS, RNA seq, etc. check the following:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326170/

Article RNA Sequencing and Analysis

Article A practical guide to single-cell RNA-sequencing for biomedic...