Will you prefer taqman or sybrgreen RTPCR?

Have you seen any difference in Taqman system and Sybrgreen RTPCR? Former is way expensive?

11 December 2017 5,505 0 View

Is Predatory Journals Omnics publisher is obslete?

31 December 2016 1,640 0 View

How we can change climate?

The conference of climate change is underway- How demonstrations are helping them? Though it is each one's responsibility. Are these demonstrations political or real people?

10 November 2015 2,852 1 View

What you think about the punishment to Dr. Hunt on his comments of females?

Sir Tim Hunt, a nobel laureate was severely punished and deprived of his whole life hard work and career reputation for the comment he made, he claimed that he did a joke only. As human beings...

06 July 2015 4,171 3 View

Which is good working antibody for MMP13 specific for human and rat for immunohistochemistry?

If someone know good working antibody for MMP13 specific for human and rat, pl share the catalog number. I appreciate, Thanks, Subhash

06 July 2015 4,225 7 View

Is there ana nti-Lubricin antibody with cross reactivity to human or human plus rat?

04 May 2015 7,717 5 View

Anyone suggest me for free software for drawing chemical structures for MAC desktop?

This question is towards organic chemistry/molecular biology researchers. I am looking for basic chemical structures drawing free software for Mac preferably. You may suggest for PC also. Thanks...

01 February 2015 7,557 6 View

Do you check and respond office e-mails after hours?

01 February 2015 6,245 5 View

What is immediate reaction to negative results of a big experiment?

I like you to share what is your immediate reaction to negative results of a big experiment which are contrary to your expectations. How you deal with it.

31 December 2014 1,427 9 View

Why all journals don't follow one universal style of research publication?

Every journal has different rules of writing style, bibliography style, figures presentation, number of references, number of figures and number of tables, number of pages limit etc. All this is...

11 December 2014 4,934 13 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

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17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View