Will you prefer taqman or sybrgreen RTPCR?

Have you seen any difference in Taqman system and Sybrgreen RTPCR? Former is way expensive?

11 December 2017 5,505 0 View

Is Predatory Journals Omnics publisher is obslete?

31 December 2016 1,640 0 View

How we can change climate?

The conference of climate change is underway- How demonstrations are helping them? Though it is each one's responsibility. Are these demonstrations political or real people?

10 November 2015 2,852 1 View

What you think about the punishment to Dr. Hunt on his comments of females?

Sir Tim Hunt, a nobel laureate was severely punished and deprived of his whole life hard work and career reputation for the comment he made, he claimed that he did a joke only. As human beings...

06 July 2015 4,171 3 View

Is there ana nti-Lubricin antibody with cross reactivity to human or human plus rat?

04 May 2015 7,717 5 View

Anyone suggest me for free software for drawing chemical structures for MAC desktop?

This question is towards organic chemistry/molecular biology researchers. I am looking for basic chemical structures drawing free software for Mac preferably. You may suggest for PC also. Thanks...

01 February 2015 7,557 6 View

Do you check and respond office e-mails after hours?

01 February 2015 6,245 5 View

What is immediate reaction to negative results of a big experiment?

I like you to share what is your immediate reaction to negative results of a big experiment which are contrary to your expectations. How you deal with it.

31 December 2014 1,427 9 View

Why all journals don't follow one universal style of research publication?

Every journal has different rules of writing style, bibliography style, figures presentation, number of references, number of figures and number of tables, number of pages limit etc. All this is...

11 December 2014 4,934 13 View

Do you prefer blind review process of your research paper?

Some journals get their research reviewed after hiding the names of the authors. What is your preference and why? What is the fair review process: blind or open

10 November 2014 8,526 14 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to classify strictly upper triangular matrices by similarity?

Let F be a field. Consider U, the set of n times n strictly upper triangular matrices in F. For X, Y in U, we call them similar if there exists some S, which is non-degenerate and upper...

01 March 2021 2,957 8 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View