Hi, I am running a ChIPseq protocol. After the IP and decrosslinking step, I add PB buffer from MinElute kit. I always need to adjust the pH with sodium acetate ( 3M, pH =5.0) to bring back the color of the PB-DNA mixture to yellow (
Hi, I am doing a systematic literature review for my MPhil. Does anyone know what philosophy/research paradigm is a systematic review?
18 June 2024 9,525 1 View
Hi I am a student in the UK, want to do my PhD research in prison in the UK interviewing Polish people in Polish language do you have any idea how I could facilitate this? How to get access to the...
14 February 2024 8,599 5 View
I want to test few 5'UTRS and 3'UTRs on their effect on luciferase expression. I will be performing in-vitro transcription to generate mRNA. I am using a luciferase vector with T7 promoter and...
07 December 2023 7,033 1 View
Hi, I plan to conduct detailed research on the endophytic microbiome in one of Apiaceae members. I will collect root, leaf and seed samples to study endophytic microbiome. I will also analyse soil...
05 December 2023 5,065 0 View
I would like to kindly ask for help. I need to prepare great amount of Dnase I solution out of the premade stock (c.a 45 ml of 40U/ml). What is the best composistion of diluting solution? I read...
03 December 2023 1,545 0 View
In ATCC this cell line is also described as CRL-1803.
25 October 2023 7,741 1 View
Hello, I'm looking for how much energy I need to evaporate 1 mole of NH3 from its aq. solution (for example 6%, at STP ) or how can I calculate this energy? To be honest, I'm not sure If this...
31 August 2023 9,930 0 View
please help me to find a software to measure distances - areas and angles in scans of thin-sections, ie flat pictures... in my case sectioned were fossil corals... 1 I have resolution and size of...
12 June 2023 9,804 1 View
I have powdered biomass that I want to analyse for heavy metal content. However, the biomass is contaminated by soil residues, which may contribute to the overall heavy metal content of the...
11 June 2023 4,151 0 View
I'm looking for the best choice of ready to use culture media for human endothelial cells isolated from aorta?
25 March 2023 4,746 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View