Is there any interaction equivalent of Cochran's Q test (like 2x3 design)?

I have data on the effectiveness of the three treatments: T1, T2 and T3 for each patient. Each variable is coded dichotomously - 0 = drug not working; 1 = drug is working. The patient could feel...

30 November 2020 1,146 14 View

Two pair of peaks of quinone/hydroquinone redox probe on GCe?

Hello, I recently stumbled upon a voltammogram of HQ/Q in 0.1M NaCl on glassy carbon electrode. I was surprised to see two pairs of peaks present on the graph. As far as I know it is a two...

29 June 2020 1,833 2 View

Non-equal variances t-test in SPSS - it's Welch or Cochran-Cox?

Can anyone help me answer the question of whether t test for unequal variances is calculated in SPSS according to Welch or Cochran-Cox correction? Test statistics are the same, they differ only...

29 May 2019 7,445 14 View

Is there any reason why one can not reduce dimensions with PCA/Alpha before putting them into SEM model?

Suppose I have three measurable variables (A, B, C on figure below) and one latent (L) in my SEM model. This one latent one is a construct/dimension consisting of 5 questions from some...

07 April 2019 905 6 View

What is precipitated in buffer solution stored in glass bottle?

Does anyone know what is precipitated in carbonate buffer solution stored in typical amber glass bottle (picture is an attachment)? The carbonate buffer in the bottle is Na2CO3/NaHCO3, pH=10.0...

06 February 2019 7,556 3 View

How many animals are required for experiment?

I want to compare 4 different methods of bladder cancer induction and I have to calculate sample size. How many animals I have to used to get appropriate statistical power?

25 November 2018 151 2 View

How to generate average contact maps from molecular dynamics?

Hi, I am looking for a way to generate a contact map between each residue of a protein during MD simulations. I.e. for each pair of residues, count the number of frames during which they form...

05 May 2018 2,023 8 View

How to get rid of a primer smear in pcr (negative control problem)?

Hello everyone My pcr was doing just fine for few months till about 3 weeks ago I started to get smears in all my samples and positive and negative controls as well. I'm preparing my reaction...

21 March 2018 2,405 7 View

How can I cryopreservate Human Hepatic Stellate Cells?

Dear all, Does anyone know or have an experience in freezing (cryopreservation) of the primary Human Hepatic Stellate Cells? How much DMSO is recommended to use? I read that cryopreservation of...

03 March 2018 9,712 2 View

Freezing atoms in gaussian - how to do it?

Hello, I'm working on a structure with about 200 atoms. It is important for me to freeze a significant part of it for the time of optimization so that the arrangement of the given atoms with each...

05 February 2018 649 7 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Where can I find the information about steel final production in Japan?

I am doing research about the in-use stock of iron and steel in Japan. I need the report about annual yield of steel final production. I found some information in the webpage of Japan Iron and...

25 January 2021 3,867 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View