Should I replace SV40 poly(A) signal with my 3'UTR of choice?

Tomasz Zbigniew Tomkiewicz @Tomasz-Tomkiewicz

08 December 2023 1 7K Report

I want to test few 5'UTRS and 3'UTRs on their effect on luciferase expression. I will be performing in-vitro transcription to generate mRNA. I am using a luciferase vector with T7 promoter and SV40 poly(A) signal. Should I clone my 3'UTR downstream from the stop codon of luciferase and upstream of SV40? Or should my 3UTR completely replace the SV40?

I am using restriction digestion for cloning.

Marion Thauvin

Hi, did you find an answer to this question ? Because I'm actually facing this problematic!

Many thanks in advance!

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