Do you always need a pH adjustement prior to MinElute purification of IPed DNA in ChIPseq?

Hi, I am running a ChIPseq protocol. After the IP and decrosslinking step, I add PB buffer from MinElute kit. I always need to adjust the pH with sodium acetate ( 3M, pH =5.0) to bring back the...

17 June 2020 4,974 0 View