What is the best culture medium for primary human endothelial cells culture ?

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Research paradigm of systematic review?

Hi, I am doing a systematic literature review for my MPhil. Does anyone know what philosophy/research paradigm is a systematic review?

18 June 2024 9,525 1 View

How to do research on Polish people in the UK prison?

Hi I am a student in the UK, want to do my PhD research in prison in the UK interviewing Polish people in Polish language do you have any idea how I could facilitate this? How to get access to the...

14 February 2024 8,599 5 View

Should I replace SV40 poly(A) signal with my 3'UTR of choice?

I want to test few 5'UTRS and 3'UTRs on their effect on luciferase expression. I will be performing in-vitro transcription to generate mRNA. I am using a luciferase vector with T7 promoter and...

07 December 2023 7,033 1 View

What homogenisation & DNA extraction method/kit should I use to perform metabarcoding of endophytic plant microbiome (roots, leaves, seeds, soil)?

Hi, I plan to conduct detailed research on the endophytic microbiome in one of Apiaceae members. I will collect root, leaf and seed samples to study endophytic microbiome. I will also analyse soil...

05 December 2023 5,065 0 View

Dnase I working conditions ?

I would like to kindly ask for help. I need to prepare great amount of Dnase I solution out of the premade stock (c.a 45 ml of 40U/ml). What is the best composistion of diluting solution? I read...

03 December 2023 1,545 0 View

Does TT cell line (medullary thyroid cancer cell line) express VEGF receptor ?

In ATCC this cell line is also described as CRL-1803.

25 October 2023 7,741 1 View

What is enthalpy of NH3 vaporation (kJ/mol) from its aq. solution?

Hello, I'm looking for how much energy I need to evaporate 1 mole of NH3 from its aq. solution (for example 6%, at STP ) or how can I calculate this energy? To be honest, I'm not sure If this...

31 August 2023 9,930 0 View

How to measure scanned thin-sections?

please help me to find a software to measure distances - areas and angles in scans of thin-sections, ie flat pictures... in my case sectioned were fossil corals... 1 I have resolution and size of...

12 June 2023 9,804 1 View

How to separate sand from pulverised biomass?

I have powdered biomass that I want to analyse for heavy metal content. However, the biomass is contaminated by soil residues, which may contribute to the overall heavy metal content of the...

11 June 2023 4,151 0 View

Which method of exosomes isolation from culture media is better?

My experiments require me to isolate exosomes from cell culture and then isolate total RNA from this exosomes. I consider 2 options to use exoquick system or invitrogen total exosome isolation...

25 March 2023 5,225 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View