Usually people add M-CSF or L929 conditioned medium to regular medium (e.g. DMEM + 10% FBS) to differentiate bone marrow into BMDM (bone marrow derived macrophages) for 7 days.
I have an argument with my friends about the use of M-CSF (or L929 medium ) in the following cell treatments.
Do you use regular medium (DMEM or RPMI + 10% FBS) in the following BMDM treatments or regular medium plus M-CSF (or L929 conditioned medium)? We found the curves are different with different medium.
Thank you so much for any input.