Sreeram Udayan

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Knockout tested western blot antibody for mice TLR2?

Not all antibodies can be used for knockout confirmation since some WB antibodies can bind to both WT and knockout equally (since the antibody recognizes nonmutated regions from the knockout)....

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Can anyone provide with a protocol for intracellular staining of cytokines: TNFa, IL-6, IL-12 in RAW264.7 murine macrophages, for flow cytometry?

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