6 Questions 31 Answers 0 Followers
Questions related from Xianfeng Wang
Usually people add M-CSF or L929 conditioned medium to regular medium (e.g. DMEM + 10% FBS) to differentiate bone marrow into BMDM (bone marrow derived macrophages) for 7 days. I have an argument...
31 March 2016 3,255 11 View
I am treating cells with all kinds of compounds, and hope to detect protein X enzymatic activity with cell lysate. But protein X has many family members (X, X1, X2, X3,.....Xn) which use...
13 November 2015 5,269 7 View
Up till now, there are two kinds of 2x Laemmli sample buffers: Buffer 1) 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue Please refer to the original...
14 May 2015 5,359 7 View
Here is my protocol: 1. Collect small intestine from mice and push out the stool. 2. Flash freeze in liquid nitrogen and then store the tissue at -80C for later use. 3. Homogenize tissues in RIPA...
28 March 2014 5,652 8 View
There are many kinds of BSA from Sigma. What BSA is good for blocking in phosph...
24 July 2013 9,852 0 View
Usually there are several (even more than 10 or 20) antibodies for a protein in the market but how do you know which antibody is better or best for your experiment? Just try it one by one?...
05 July 2013 6,111 24 View