Xianfeng Wang

6 Questions 31 Answers 0 Followers

Questions related from Xianfeng Wang

Do you add M-CSF or L929-conditioned medium to cell culture when you treat BMDM cells?

Usually people add M-CSF or L929 conditioned medium to regular medium (e.g. DMEM + 10% FBS) to differentiate bone marrow into BMDM (bone marrow derived macrophages) for 7 days. I have an argument...

31 March 2016 3,256 11 View

How do I isolate an endogenous enzyme to detect its activity?

I am treating cells with all kinds of compounds, and hope to detect protein X enzymatic activity with cell lysate. But protein X has many family members (X, X1, X2, X3,.....Xn) which use...

13 November 2015 5,270 7 View

What 2x Laemmli Sample Buffer recipe is better?

Up till now, there are two kinds of 2x Laemmli sample buffers: Buffer 1) 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue Please refer to the original...

14 May 2015 5,360 7 View

Can anybody help me with western blot using small intestine tissue lysate?

Here is my protocol: 1. Collect small intestine from mice and push out the stool. 2. Flash freeze in liquid nitrogen and then store the tissue at -80C for later use. 3. Homogenize tissues in RIPA...

28 March 2014 5,653 8 View

Which BSA is good for Western Blot with anti-phosph antibody?

There are many kinds of BSA from Sigma. What BSA is good for blocking in phosph...

24 July 2013 9,853 0 View

How do you choose a good antibody for western blot?

Usually there are several (even more than 10 or 20) antibodies for a protein in the market but how do you know which antibody is better or best for your experiment? Just try it one by one?...

05 July 2013 6,112 24 View