Is there a short tandem repeat (STR) database for mouse cell line authentification?

Hi, Have a mystery cell line of mouse origin that I need help identifying. I used the IDEXX service for STR analysis but the results were inconclusive. Seems like the database is forthcoming but...

03 April 2019 6,584 1 View

How would you distinguish granulocytic myeloid derived suppressor cells from a neutrophil?

The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.

02 March 2016 7,017 4 View

Are 14M1.4 macrophage cells publicly available?

10 November 2014 1,507 0 View

Is hair a problem with NIR dylight 747 whole body imaging?

08 September 2014 3,974 3 View

What is your favorite Elisa/antibody pairs for mil-18?

07 August 2014 3,956 0 View

IL-1B, caspase 1, caspase 11 antibodies for western blot of murine samples?

Looking for a good primary antibody.

10 November 2013 7,856 2 View

BFA i.v. injection insolubility

I would like to inject 250 ug of BFA i.v. into a mouse and at the dilution step from neat DMSO to 10% DMSO in PBS the BFA crashes out of solution (BFA is at 1.25 mg/ml at this point). Usually...

06 July 2013 2,951 1 View

Has anyone managed to successfully lower the number of beads for luminex detection of cytokines?

After seeing how little of the sample is consumed to acquire the desired number of beads, I thought we may be able to stretch the kit by lowering the number of beads per sample. Hopefully this...

05 June 2013 968 11 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View