Di0c6 and fixation?

Anyone know if di0c6 can be used before PFA fixation?

31 December 2017 1,677 2 View

How would you distinguish granulocytic myeloid derived suppressor cells from a neutrophil?

The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.

02 March 2016 7,017 4 View

Are 14M1.4 macrophage cells publicly available?

10 November 2014 1,507 0 View

Is hair a problem with NIR dylight 747 whole body imaging?

08 September 2014 3,974 3 View

What is your favorite Elisa/antibody pairs for mil-18?

07 August 2014 3,956 0 View

IL-1B, caspase 1, caspase 11 antibodies for western blot of murine samples?

Looking for a good primary antibody.

10 November 2013 7,856 2 View

BFA i.v. injection insolubility

I would like to inject 250 ug of BFA i.v. into a mouse and at the dilution step from neat DMSO to 10% DMSO in PBS the BFA crashes out of solution (BFA is at 1.25 mg/ml at this point). Usually...

06 July 2013 2,951 1 View

Has anyone managed to successfully lower the number of beads for luminex detection of cytokines?

After seeing how little of the sample is consumed to acquire the desired number of beads, I thought we may be able to stretch the kit by lowering the number of beads per sample. Hopefully this...

05 June 2013 968 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View