Are 14M1.4 macrophage cells publicly available?

11 November 2014 0 2K Report

I'm looking for this macrophage cell line and would greatly appreciate help.

Badges
Science topic

More Anthony Desbien's questions See All

Is there a short tandem repeat (STR) database for mouse cell line authentification?

Hi, Have a mystery cell line of mouse origin that I need help identifying. I used the IDEXX service for STR analysis but the results were inconclusive. Seems like the database is forthcoming but...

03 April 2019 6,673 1 View

Di0c6 and fixation?

Anyone know if di0c6 can be used before PFA fixation?

31 December 2017 1,758 2 View

How would you distinguish granulocytic myeloid derived suppressor cells from a neutrophil?

The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.

02 March 2016 7,117 4 View

Is hair a problem with NIR dylight 747 whole body imaging?

I need to image mice with a whole animal imaging platform and I'm wondering if it is necessary to remove hair with dylight747. I'll be image c57bl6.

08 September 2014 4,049 3 View

What is your favorite Elisa/antibody pairs for mil-18?

Il-18 detection. I was using Ebio's but they've stopped production.

07 August 2014 4,044 0 View

IL-1B, caspase 1, caspase 11 antibodies for western blot of murine samples?

Looking for a good primary antibody.

10 November 2013 7,947 2 View

BFA i.v. injection insolubility

I would like to inject 250 ug of BFA i.v. into a mouse and at the dilution step from neat DMSO to 10% DMSO in PBS the BFA crashes out of solution (BFA is at 1.25 mg/ml at this point). Usually...

06 July 2013 3,046 1 View

Has anyone managed to successfully lower the number of beads for luminex detection of cytokines?

After seeing how little of the sample is consumed to acquire the desired number of beads, I thought we may be able to stretch the kit by lowering the number of beads per sample. Hopefully this...

05 June 2013 1,054 11 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View