I need to image mice with a whole animal imaging platform and I'm wondering if it is necessary to remove hair with dylight747. I'll be image c57bl6.
The intensity of the registered signal will be significantly reduced by both fur and skin. It means, that even if you remove fur carefully you will have reduction of signal by skin (white skin is a little bit better for light penetration than black one). Please, also take into account, that even small rest of the fur in the field of view will bring admixture in the image like small cloud and each wound (cut) will be a source of bright fluorescence as if you take out the skin in this place. So, the level of the registered signal from the same s.c. placed fluorescent sample will reduced along the line: mice without skin (for example, autopsy), hairless mice, mice with white fur after shaving, mice with black fur after shaving, mice with white fur, mice with black fur.
