How to incorporate a variable in damage model?

15 December 2020 7,284 2 View

Mathematical or computational modelling of protein oxidation?

I am looking for a research paper about the mathematical or computational modelling of protein oxidation (caused by reactive oxygen species).. I would really appreciate that if someone helps me...

17 November 2020 2,340 3 View

Can FEM model learn from experimental results?

I am looking for a research in which the finite element model was trained based on experimental results.

02 October 2020 9,277 19 View

Master thesis about the field of the luxury watch market?

18 September 2020 5,802 5 View

For connection between the bolt to the steel element, which kind of interaction should be considered on ABAQUS?

This design is to check for rupture in the steel element and the behavior of the bolt does not matter.

14 August 2020 6,580 2 View

Is it possible for anybody to help me for fix bridge modeling in OPENSEES?

I modeled a two-span concrete box girder bridge base on FHWA design specification, and now I recognize a problem in modeling because my first period is higher than I expected. With initial...

12 July 2020 415 3 View

Why does stresses evolve in only one element in Abaqus explicit?

I am trying to run my VUMAT code with a model in Abaqus/explicit. After that the run has been completed, I observed that the stresses have been evolved in only one element as shown in the attached...

27 May 2020 5,506 3 View

How to do baseline correction for DSC test data?

I have some raw DSC test data and I need to do baseline correction. I don't know how should I do it. I would appreciate it if someone can help me with this.

16 May 2020 8,273 7 View

Problem with nonlinear curve fitting?

Hello all, Actually, I am dealing with an issue that I can't get desirable results out of my curve-fitting problem. I used lsqcurvefit in the first place. Then, I added MultiStart to my code as...

30 April 2020 4,943 2 View

Tips on how to implement organic computing?

I'm researching the Self - Adaptive System of hospital systems.But I've had trouble figuring out how to do organic computing for self-adaptation in hospital systems.I am looking for an article or...

14 April 2020 1,743 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View