Why knockouts for same genes from two different gRNA are showing different result?
I followed CRISPR-CAS9 gene editing technology.
Even the same gRNA will produce different repair events. It is likely that the repair events/sequence derived from each of your experiments differ from each other, hence why they 'show a different result'.
I am designing a DAB DC DC Converter and when I am connecting a battery block in Matlab and fix its specification lets say 350 V and 25 A, when I am giving input as 600V, Battery would charge to...
11 February 2021 3,110 1 View
I am trying to model a DAB converter and I need to observe the power transfeered to the load side on varying the phase shift between two bridges, hence I couldnt take a constant load e.g...
09 February 2021 6,489 3 View
Purified G actin and got 42kda band and an additional band just below it. While performing G actin polymerization and labeling with rhodamine-phalloidin I didn't observe filaments under the...
20 January 2021 3,751 2 View
I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...
05 January 2021 9,882 3 View
Also if anyone can share latest research regarding Geo-disaster of quick clay except in Norway? Thanks Kind Regards Dr. Khan
05 January 2021 4,645 3 View
The RNA quantity in nanodrop is about 1 microgm. 260/280 ratio is also around 2, still I am not getting clear bands on gel. What can be the reason?
30 December 2020 1,731 2 View
here I'm asking about different variants of 3D software's used to get STL file measurements made from CBCT
20 December 2020 4,450 2 View
I have read one research article by M Dieterle et al. In this article they have discussed about finding stoichiometry of MoO3-x using DR UV-Vis spectra. I am not getting the required equation they...
18 December 2020 9,755 2 View
Hello, I need to plot Taylor diagrams for some of my analysis. However, I am not sure how can I plot them either in excel or Matlab. I have searched on several sites, I could not reach any final...
17 December 2020 5,228 3 View
I want to find the fetal brain MRI dataset for cnn model and deep learning.Can anyone help me, thank you very much
09 December 2020 9,966 2 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
23 February 2021 8,969 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View
23 February 2021 8,850 2 View
Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...
22 February 2021 5,341 5 View