It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Article Genome-wide CRISPR Screens in Primary Human T Cells Reveal K...
)Given cells were cultured at 1E6 cells/mL (that is, 400 mL), 2 mL per well in 6-well plates, it seemed that I have to prepare 200 wells (approximately 34 6-well plates). I wonder if there is better way to do that.
Of note, I have to use plate-bound antibody rather than beads, which was explained in their article.
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