I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible targeting events or if I should do flow to select positive cells. Any suggestion? Thank you for any help.
Flávia Caroline Gan
It depends. If you just want to separate your pool of GFP-positive cells and if fluorescence is always being expressed, flow is sufficient.
Single clones are when you need a specific population. The result is much more accurate, but the work is greater.
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