For example, If a PCR primer can detect ~100 fg of DNA, how we will calculate it to Bacterial copy number?
Depends on the size of the bacterial genome and if they have any extrachromosomal elements like plasmids.
Here's a formula you can use if your bacterial genome is 4.6 Mbp
Copy no. = {(fg of DNA x 6.022x10^23 copies/mol)/(4.6x10^6 bp x660 g/mol/bp)}
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