How much is complete DNA genome Pichia pastoris?
Can anyone help me with a heterologous protein purification problem from Pichia pastoris supernatant? I am trying to purify the 59.6 kDa protein from the supernatant (protein has secretion...
11 December 2017 9,344 0 View
Is it necessary to rate 260 to 280 plasmid after digested to be 1.8 then do transformation Pichia pastoris by electroporation? I use Phenol: chloroform: isoamyl alcohol, but I cant get suitable...
09 October 2017 3,542 4 View
What is the simple method extraction DNA from Pichia pastoris?
09 October 2017 7,475 0 View
What is the best way to purify the digested product?Is there another method other than ethanol purification?
07 August 2017 2,386 1 View
how can remove the endonuclease E. coli in plasmid extraction?
07 August 2017 972 4 View
how much DNA need for digest? The construction plasmid is 4700 ng/µL, I want linear it with Fermentas restriction enzyme (Stu I). and what is the best method for extract vector after digest?
07 August 2017 2,349 6 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
06 July 2017 7,005 6 View
what is a role transcription termination(TT) fragment in the Phil_s1 plasmid?
05 June 2017 1,823 0 View
I want to do a virtual clone. What do you recommend free software for the virtual clone?
05 June 2017 7,195 2 View
I want to Codon optimization of Thermobifidia fusca cel6B gene and its expression in Pichia pastoris. How can to do it?
31 December 2015 6,861 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
