What is the simple method extraction DNA from Pichia pastoris?
Can anyone help me with a heterologous protein purification problem from Pichia pastoris supernatant? I am trying to purify the 59.6 kDa protein from the supernatant (protein has secretion...
11 December 2017 9,344 0 View
Is it necessary to rate 260 to 280 plasmid after digested to be 1.8 then do transformation Pichia pastoris by electroporation? I use Phenol: chloroform: isoamyl alcohol, but I cant get suitable...
09 October 2017 3,542 4 View
What is the best way to purify the digested product?Is there another method other than ethanol purification?
07 August 2017 2,386 1 View
how can remove the endonuclease E. coli in plasmid extraction?
07 August 2017 972 4 View
how much DNA need for digest? The construction plasmid is 4700 ng/µL, I want linear it with Fermentas restriction enzyme (Stu I). and what is the best method for extract vector after digest?
07 August 2017 2,349 6 View
How much is complete DNA genome Pichia pastoris?
06 July 2017 6,090 0 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
06 July 2017 7,005 6 View
what is a role transcription termination(TT) fragment in the Phil_s1 plasmid?
05 June 2017 1,823 0 View
I want to do a virtual clone. What do you recommend free software for the virtual clone?
05 June 2017 7,195 2 View
I want to Codon optimization of Thermobifidia fusca cel6B gene and its expression in Pichia pastoris. How can to do it?
31 December 2015 6,861 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View