Methods precipitation

Karim Imangholiloo @Karim_Imangholiloo

08 August 2017 1 2K Report

What is the best way to purify the digested product?

Is there another method other than ethanol purification?

Karim Imangholiloo

Thank you, Dear Shokrian.

Badges
Science topic

More Karim Imangholiloo's questions See All

Problem hetrologous protien purification Pichia pastoris?

Can anyone help me with a heterologous protein purification problem from Pichia pastoris supernatant? I am trying to purify the 59.6 kDa protein from the supernatant (protein has secretion...

11 December 2017 9,344 0 View

Effect rate 260 to 280 in transformation pichia?

Is it necessary to rate 260 to 280 plasmid after digested to be 1.8 then do transformation Pichia pastoris by electroporation? I use Phenol: chloroform: isoamyl alcohol, but I cant get suitable...

09 October 2017 3,542 4 View

Extraction DNA Pichia?

What is the simple method extraction DNA from Pichia pastoris?

09 October 2017 7,475 0 View

Remove enzyme when plasmid extraction?

how can remove the endonuclease E. coli in plasmid extraction?

07 August 2017 972 4 View

Digest with fermentas enzyme and best method for extract product digested

how much DNA need for digest? The construction plasmid is 4700 ng/µL, I want linear it with Fermentas restriction enzyme (Stu I). and what is the best method for extract vector after digest?

07 August 2017 2,349 6 View

Complete DNA Genome Pichia

How much is complete DNA genome Pichia pastoris?

06 July 2017 6,090 0 View

Extract DNA genomic fungi

How can extract DNA genomic with high purity in fungal with the CTAB method?

06 July 2017 7,005 6 View

Role transcription termination

what is a role transcription termination(TT) fragment in the Phil_s1 plasmid?

05 June 2017 1,823 0 View

Virtual clone

I want to do a virtual clone. What do you recommend free software for the virtual clone?

05 June 2017 7,195 2 View

How to optimize codon usage?

I want to Codon optimization of Thermobifidia fusca cel6B gene and its expression in Pichia pastoris. How can to do it?

31 December 2015 6,861 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...

23 July 2024 3,490 6 View