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Questions related from Karim Imangholiloo
Can anyone help me with a heterologous protein purification problem from Pichia pastoris supernatant? I am trying to purify the 59.6 kDa protein from the supernatant (protein has secretion...
12 December 2017 9,354 0 View
What is the simple method extraction DNA from Pichia pastoris?
10 October 2017 7,493 0 View
Is it necessary to rate 260 to 280 plasmid after digested to be 1.8 then do transformation Pichia pastoris by electroporation? I use Phenol: chloroform: isoamyl alcohol, but I cant get suitable...
10 October 2017 3,556 4 View
how much DNA need for digest? The construction plasmid is 4700 ng/µL, I want linear it with Fermentas restriction enzyme (Stu I). and what is the best method for extract vector after digest?
08 August 2017 2,365 6 View
how can remove the endonuclease E. coli in plasmid extraction?
08 August 2017 997 4 View
What is the best way to purify the digested product?Is there another method other than ethanol purification?
08 August 2017 2,402 1 View
How much is complete DNA genome Pichia pastoris?
07 July 2017 6,102 0 View
How can extract DNA genomic with high purity in fungal with the CTAB method?
07 July 2017 7,017 6 View
I want to do a virtual clone. What do you recommend free software for the virtual clone?
06 June 2017 7,221 2 View
what is a role transcription termination(TT) fragment in the Phil_s1 plasmid?
06 June 2017 1,844 0 View
I want to Codon optimization of Thermobifidia fusca cel6B gene and its expression in Pichia pastoris. How can to do it?
01 January 2016 6,869 2 View
