Virtual clone - FAQS.TIPS

More Karim Imangholiloo's questions See All

Problem hetrologous protien purification Pichia pastoris?

Can anyone help me with a heterologous protein purification problem from Pichia pastoris supernatant? I am trying to purify the 59.6 kDa protein from the supernatant (protein has secretion...

11 December 2017 9,344 0 View

Effect rate 260 to 280 in transformation pichia?

Is it necessary to rate 260 to 280 plasmid after digested to be 1.8 then do transformation Pichia pastoris by electroporation? I use Phenol: chloroform: isoamyl alcohol, but I cant get suitable...

09 October 2017 3,542 4 View

Extraction DNA Pichia?

What is the simple method extraction DNA from Pichia pastoris?

09 October 2017 7,475 0 View

Methods precipitation

What is the best way to purify the digested product?Is there another method other than ethanol purification?

07 August 2017 2,386 1 View

Remove enzyme when plasmid extraction?

how can remove the endonuclease E. coli in plasmid extraction?

07 August 2017 972 4 View

Digest with fermentas enzyme and best method for extract product digested

how much DNA need for digest? The construction plasmid is 4700 ng/µL, I want linear it with Fermentas restriction enzyme (Stu I). and what is the best method for extract vector after digest?

07 August 2017 2,349 6 View

Complete DNA Genome Pichia

How much is complete DNA genome Pichia pastoris?

06 July 2017 6,090 0 View

Extract DNA genomic fungi

How can extract DNA genomic with high purity in fungal with the CTAB method?

06 July 2017 7,005 6 View

Role transcription termination

what is a role transcription termination(TT) fragment in the Phil_s1 plasmid?

05 June 2017 1,823 0 View

How to optimize codon usage?

I want to Codon optimization of Thermobifidia fusca cel6B gene and its expression in Pichia pastoris. How can to do it?

31 December 2015 6,861 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Why do open and free science in a world where science is not open and free?

Because I have realized that the world tends more and more to do open and free science and there is a trend more and more to choose free databases, free tools and open access platforms.

01 August 2024 10,046 1 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View