Co-transfection of miRNA mimics and inhibitors?

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Transfected cells died after trypsinization ?

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Constitutive expression of eGFP in transfected cells?

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New in lentivirus transduction work?

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Problem with stable cell line generation?

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Will miRNA transfection affect cell morphology?

Hi Scientist, I am transfecting an oncogenic miRNA into a breast cancer cell line and based on your experience in the mammalian cell line, will such transfection affect the cell morphology?

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Suggestion for good plasmid extraction kit?

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MiRNA primer sequence?

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Use of fluorescence or luciferase approach in checking transfection efficiency?

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Single plasmid that carries both miRNA and reporter gene like GFP/luciferase?

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Viral or non-viral constructs for transfection?

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Anyone having idea about VN primer for miRNA primer design ?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Transfection in HEK293T cells?

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Issues with Protein Expression in Transfected cells?

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Can i use the protease inhibitors during cell membrane vesicle preparation ？?

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How to retain the a GFP tagged gene expression in stable cell line?

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Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

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Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

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Seeking recommendations for equipment to mimic sunlight and heat up a greenhouse system?m?

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Can I use Polyjet after its expiration date?

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Andreas Eisenreich

I would propose to perform a literature search via NCBI Pubmed to answer your question.

Alexander Sasha Vlassov

for the same miRNA target? never saw such a paper, everyone is transfecting either mimic or inhibitor- separately, so that you can either increase or decrease specific miRNA levels.

Zx Chong

Yes Dr. Alexander Sasha Vlassov . I was recommended by some people on transfecting both miRNA mimics and inhibitors in the same cell.

You are right. It is hard for me to find papers that reported co-transfection of mimics and inhibitor in the same cell.