Hi all,
I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell contact, in hoping the cells can proliferate better. But every time after I trypsinize them, the transfected cells would die afterwards.
I have also included untransfected (normal) cells during the trypsinization and the normal cells do not die and proliferate well after trypsinization and well transferring.
Any way to solve this issue?