09 September 2019 2 9K Report

Hi all,

I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are also get killed. I am not sure why my transfected cells could not survive. Apart from lowering the antibioctics concentration, do you have any suggestion for me to troubleshoot this problem?

Thank you.

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