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Questions related from Zx Chong
Hi all, I am facing a problem to trypsinize and combine/move the transfected cells into new well . I was planning to pool the transfected cells into a new well to ensure better cell-cell...
12 December 2019 3,385 5 View
Hi, I am trying to culture a stable clones using transfected cells. As the clones grow, I noticed the eGFP signal diminishes over time but the cells are still resistant to antibiotics and do not...
12 December 2019 305 14 View
Hi all, I am new in lentivirus transduction work and have some problem as listed below: 1) Is CMV promoter considered a strong promoter to express the gene I want in the transgenic organism...
10 October 2019 8,708 4 View
Hi all, I am facing a problem to generate a stable cell line after successful transfection. I added puromycin to kill the untransfected cells, however, eventually all my transfected cells are...
09 September 2019 9,136 2 View
Hi, Can anyone recommend a plasmid extraction kit that could produce good plasmid yield (>300ng/uL). The plasmid I am using now is a high copy number plasmid and the bacteria I am using is...
07 July 2019 6,937 3 View
Hi Scientist, I am transfecting an oncogenic miRNA into a breast cancer cell line and based on your experience in the mammalian cell line, will such transfection affect the cell morphology?
07 July 2019 7,524 8 View
Dear all, I have a miRNA primer sequence left by someone else and I would like to know about the sequence of this primer. Is there any method I can use to sequence and know the sequence of this...
06 June 2019 6,129 7 View
Hi, Is there any paper which reported the co-transfection of miRNA mimics and inhibitors? Thank you.
06 June 2019 9,831 3 View
Hi all, I would like to seek for your opinions on whether fluorescence approach (GFP) or luciferase approach is better in assessing transfection efficiency / success (after miRNA...
04 April 2019 1,506 6 View
Hi all, I would like to ask your opinion on whether viral or non-viral construct is better and more superior for transfection involving human breast cancer cell lines, MDA-MB-231? Besides, in...
04 April 2019 6,484 4 View
Hi researcher, I wonder any of you have tried to design a single plasmid that carries both miRNA and reporter gene like GFP / luciferase? I am thinking with such plasmid, I will just need to...
04 April 2019 5,258 7 View
Hi all. I would like to know whether there is any published paper or script that can be used as a guide to analyze the miRNA expression data using the R studio? If you know any, please let me...
03 March 2019 7,545 4 View
Hi all, I would like to know whether I could use the same positive and negative controls for different miRNA transfection set (for example miRNA-A and miRNA-B) using the same mammalian cell...
03 March 2019 4,035 5 View
Hi all, I am planning for some transfection work involving a mammalian cancer cell line. Can I know the transfection kit from Qiagen or ThermoFisher lipofectamine is a better option? Do you...
03 March 2019 2,071 3 View
Hi all, I am facing a problem here and might need TP53 expert's opinion. I will need to amplify the DNA binding domain of TP53 (exon 5 - 9) in just 3 reactions. Based on the different published...
01 January 2019 7,903 5 View
Dear Scientist, I am planning to extract genomic DNA from human brain tissue and is there any cheap and superior conventional protocol to do so? As for the extraction kit, anyone has any good...
12 December 2018 2,549 5 View
Hi, Anyone has used the Qiagen DNA extraction kit (with the column filter) to extract DNA from human brain tissues? I am planning to extract DNA using the same kit and previously I have been...
12 December 2018 4,388 2 View
Dear Scientist, I am looking for constructive feedback on the possible causes of pale shady band below the desired band on agarose gel. Kindly refer to the photo attached and you may find pale...
12 December 2018 1,488 3 View
Hi all, Recently I have been trying to sequence a local edible mushroom strain and it was found that the results were not really good due to many background noise and I was told that this might...
02 February 2018 3,634 9 View
Hi all, I would like to ask what is the best way to present findings on the nitric oxide scavenging (inflammatory) assay? IC50, percentage of inhibtion (%) OR nitrite equivalent (compared to...
02 February 2018 2,599 0 View
Dear all, I have a question on the DNA sequencing of dikaryotic VS monokaryotic fungi. Let's say I have a fungi species (sample) and one is dikaryotic while the other one is monokaryotic. Will...
02 February 2018 944 10 View
Hi all, I have recently collected some DNA information of around 10 mushroom samples. I have done constructing the phylogenetic tree and obtained the bootstrap value using the MEGA software. My...
02 February 2018 965 3 View
In a concentration-response curve, both control and drug X almost stay at the same position and have same slopes. Is there a way to determine CR of X? I was told that X could be a competitive...
12 December 2017 9,875 0 View
Hi all, I am currently working on compounds extractions from an edible mushroom and after rounds of alcoholic (ethanolic extraction), I notice the yields was quite low. I am using 100% ethanol...
12 December 2017 7,964 0 View
There are many types of literature reviews, for example: systematic review, methodological review, integrative reviews etc. Among all these reviews, which type has a higher chance to be published...
12 December 2017 2,941 1 View
Hi all, I am working on transfection experiments involving miRNA in mammalian cell lines and I have a doubt here. After optimizing my experiment, I am able to achieve good transfection efficieny...
01 January 1970 3,907 5 View
Hi all, Recently I was recommended to use QIAGEN miRCURY LNA miRNA mimics / inhibitors in transfecting mammalian cancer cell lines. I was told that the mentioned mimics / inhibitors can be...
01 January 1970 5,482 7 View
