Hi all,
I would like to ask your opinion on whether viral or non-viral construct is better and more superior for transfection involving human breast cancer cell lines, MDA-MB-231?
Besides, in between U6 and H1 promoter, which is better to be included in the plasmid constructs?
Hope to hear from you ! Thank you in advance!
Hi. It depends on the toxicity of your viral vector, it's payload size and duration of trans-gene expression. Its better to compare these criteria among your choices.
I'm assuming your question is with regards to lentiviral transfection, and yes, it is usually more efficient than other techniques in terms of overall efficiency. However, larger constructs and potentially negative effects of viral transfection can warrant using other techniques, like lipofection (see https://altogen.com/product/mda-mb-transfection-reagent-breast-adenocarcinoma/ ). For breast cancer cell lines, the transfection efficiency of these chemical approaches can match that of viral transfections, and it can end up being easier to execute as well.
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