Molecular Dynamic Simulation error in GROMACS version 2020.1 (PME rank 2 are more than 2/3 times the cut-off). Is there any solution to this?

I'm running a protein-ligand Molecular Dynamic Simulation but getting the following error after hitting mdrun. Can anyone offer a solution to this? Thanks in advance. Fatal error: 66 particles...

03 February 2021 1,231 4 View

Can anyone suggest me a list of journals in computer science discipline those go for rapid publication (monthly issue actually)?

I would like to publish some articles rapidly. It would be so nice if you suggest me a list of journals those publishes monthly usually. Thanks in advance.

30 December 2020 4,648 1 View

What is the significance of H-Index and Eigenfactor in assessing the quality of a journal?

Its getting tough now a days to successfully assess the quality of a journal. H-Index and Eigenfactor are two such parameters used frequently to determine the quality of a journal. I would like...

29 December 2020 9,032 1 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Is there any complete documentation for HTM (Hierarchical Temporal Memory)?

Currently, I am working on implementing HTM for some of state of the arts problems. I am facing some issues actually. A complete documentation may help I think.

17 September 2020 8,875 1 View

Which formula to use to calculate the sample size for a cross sectional comparative study?

If i intend to carry out a cross sectional comparative study (analytical study design) to assess the association of cardiac diseases among young stroke patients, where do I begin? Do I enroll...

07 June 2020 5,505 5 View

What is the best method of determining amplification efficiency in qPCR system?

19 April 2020 9,019 3 View

Hexokinase (HK2) Activity Assay: Can anyone suggests me, How can i measure the activity of recombinant Hexokinase 2 enzyme?

I have clone and expressed the human Hexokinase 2 gene in Pichia pastoris.Now i want to see the activities of expressed HKII protein.Can anyone provide me protocol to measures the activity of Hk2...

25 July 2019 8,757 3 View

What are the tools to design random primer?

May I know the tools or software to design the random primer for PCR? Thanks in advance.

15 January 2019 3,367 3 View

Could anyone provide carp fin tissue-genomic DNA banding pattern after gel electrophoresis?

What is the Genomic DNA banding pattern in carp fin tissue? Is it single band or smear? Could anyone provide this information? I will be grateful.

06 January 2019 4,020 1 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View

Separated objects vs Non-Manifold Assembly?

I'm trying to do a simulation between the Maxilla and the Mandible I converted the Dicom file from the CT scan to a CAD module and module the articular discs via 3d software. I have two options...

08 February 2021 8,731 2 View

How do I apply continuity boundary condition at the edge using form assembly in comsol?

The continuity boundary condition is automatically created for a shared (common) surface boundary but I want to have it for an edge. Please help me out. In image 8 is the base of the cylinder and...

05 February 2021 3,459 2 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

Could someone please suggest a suitable program for assembling plasmids containing repetitive sequences from short paired-end reads?

Hello! I'm trying to assemble some plasmid vectors that contain two repetitive regions (with around 500 and 250 bp) and it is being hard to get the complete sequence. If someone knows a better...

03 February 2021 6,379 3 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

How can i improve my DNA purity for whole genome sequence that has high 260/230 ratio ?

I am performing whole genome sequence for gram negative acinetobacter baumannii using two platforms illumina miswq and oxford nanopore. Thus i require a very high yield and high purity. I am using...

31 January 2021 355 2 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View