Molecular Dynamic Simulation error in GROMACS version 2020.1 (PME rank 2 are more than 2/3 times the cut-off). Is there any solution to this?

I'm running a protein-ligand Molecular Dynamic Simulation but getting the following error after hitting mdrun. Can anyone offer a solution to this? Thanks in advance. Fatal error: 66 particles...

03 February 2021 1,231 4 View

Can anyone suggest me a list of journals in computer science discipline those go for rapid publication (monthly issue actually)?

I would like to publish some articles rapidly. It would be so nice if you suggest me a list of journals those publishes monthly usually. Thanks in advance.

30 December 2020 4,648 1 View

What is the significance of H-Index and Eigenfactor in assessing the quality of a journal?

Its getting tough now a days to successfully assess the quality of a journal. H-Index and Eigenfactor are two such parameters used frequently to determine the quality of a journal. I would like...

29 December 2020 9,032 1 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Is there any complete documentation for HTM (Hierarchical Temporal Memory)?

Currently, I am working on implementing HTM for some of state of the arts problems. I am facing some issues actually. A complete documentation may help I think.

17 September 2020 8,875 1 View

Which formula to use to calculate the sample size for a cross sectional comparative study?

If i intend to carry out a cross sectional comparative study (analytical study design) to assess the association of cardiac diseases among young stroke patients, where do I begin? Do I enroll...

07 June 2020 5,505 5 View

What is the best method of determining amplification efficiency in qPCR system?

19 April 2020 9,019 3 View

Hexokinase (HK2) Activity Assay: Can anyone suggests me, How can i measure the activity of recombinant Hexokinase 2 enzyme?

I have clone and expressed the human Hexokinase 2 gene in Pichia pastoris.Now i want to see the activities of expressed HKII protein.Can anyone provide me protocol to measures the activity of Hk2...

25 July 2019 8,757 3 View

What are the tools to design random primer?

May I know the tools or software to design the random primer for PCR? Thanks in advance.

15 January 2019 3,367 3 View

Could anyone provide carp fin tissue-genomic DNA banding pattern after gel electrophoresis?

What is the Genomic DNA banding pattern in carp fin tissue? Is it single band or smear? Could anyone provide this information? I will be grateful.

06 January 2019 4,020 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View

MVN, how to conciliate contradictory results from Multivariate normality and Multivariate outliers test?

Dear colleagues, I'm working with Path analyses in lavaan and MVN packages. There are some results that for me are confusing and features from the MVN that I do not know how to set. My dataset is...

18 February 2021 190 3 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View