24 July 2024 6 3K Report

Hi Everyone,

I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any similarities either. But it is causing 30-50% decrease in my target gene in two different cell lines.

Do you have any suggestions on what might be causing this?

Thank you in advance!

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