Molecular Dynamic Simulation error in GROMACS version 2020.1 (PME rank 2 are more than 2/3 times the cut-off). Is there any solution to this?

I'm running a protein-ligand Molecular Dynamic Simulation but getting the following error after hitting mdrun. Can anyone offer a solution to this? Thanks in advance. Fatal error: 66 particles...

03 February 2021 1,231 4 View

Can anyone suggest me a list of journals in computer science discipline those go for rapid publication (monthly issue actually)?

I would like to publish some articles rapidly. It would be so nice if you suggest me a list of journals those publishes monthly usually. Thanks in advance.

30 December 2020 4,648 1 View

What is the significance of H-Index and Eigenfactor in assessing the quality of a journal?

Its getting tough now a days to successfully assess the quality of a journal. H-Index and Eigenfactor are two such parameters used frequently to determine the quality of a journal. I would like...

29 December 2020 9,032 1 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Is there any complete documentation for HTM (Hierarchical Temporal Memory)?

Currently, I am working on implementing HTM for some of state of the arts problems. I am facing some issues actually. A complete documentation may help I think.

17 September 2020 8,875 1 View

Which formula to use to calculate the sample size for a cross sectional comparative study?

If i intend to carry out a cross sectional comparative study (analytical study design) to assess the association of cardiac diseases among young stroke patients, where do I begin? Do I enroll...

07 June 2020 5,505 5 View

What is the best method of determining amplification efficiency in qPCR system?

19 April 2020 9,019 3 View

Hexokinase (HK2) Activity Assay: Can anyone suggests me, How can i measure the activity of recombinant Hexokinase 2 enzyme?

I have clone and expressed the human Hexokinase 2 gene in Pichia pastoris.Now i want to see the activities of expressed HKII protein.Can anyone provide me protocol to measures the activity of Hk2...

25 July 2019 8,757 3 View

What are the tools to design random primer?

May I know the tools or software to design the random primer for PCR? Thanks in advance.

15 January 2019 3,367 3 View

Could anyone provide carp fin tissue-genomic DNA banding pattern after gel electrophoresis?

What is the Genomic DNA banding pattern in carp fin tissue? Is it single band or smear? Could anyone provide this information? I will be grateful.

06 January 2019 4,020 1 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Can Immunogenic BNT162b vaccines protect HUMANS from SARS-CoV-2?

"A safe and effective vaccine against COVID-19 is urgently needed in quantities sufficient to immunise large populations. We report the preclinical development of two BNT162b vaccine candidates,...

11 February 2021 6,628 9 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

How do I dissociate an antibody used for positive selection of cells in order to re-culture the selected cells?

Hi, I am interested in cells that express a specific receptor. I use a fluorophore-conjugated antibody bound to the receptor followed by MACS positive selection to enrich for this...

21 January 2021 2,245 3 View

Can anyone helps me with Autodock 4.2 scripts error ?

After opening the file, I operate command "Script" --> "Ligand Interactions" --> "Show Ligand Binding Site Atoms" and the warning appears as "This script displays ligands and surrounding...

15 January 2021 5,360 1 View

Do drugs that are injected i.t in rats reach the supraspinal system and how long does it take to clear them from spinal cord?

I am injecting intrathecally (between L5-L6) , in rats, cannabinoid analgesics and I was wondering if they can reach the brain and have effects there as well ? Also I need to pretreat with...

07 January 2021 5,397 4 View

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

Are the receptor proteins on the surface of infected cells will increase or decrease the susceptibility of antivirals?

22 December 2020 3,242 4 View

Autodock Raccoon - the following maps are missing?

I've generated a set of maps for my receptor using ADT (AutoDockTools). It has resulted in the files: .A.map .C.map .HD.map .N.map .NA.map .OA.map .SA.map .d.map .e.map But when I try loading the...

20 December 2020 7,721 2 View

Dock the ligand to the receptor with smina?

I docked the ligand with the molecule by smina, and got a file in mol format. However, two object ligands and protein are needed when the program is called. The mol file generated by smina has...

06 December 2020 7,342 1 View

What is the effect of harvesting lentivirus late?

Hello, I typically harvest my lentivirus in the supernatant from packaging cells 48 hours after the media change from transfection. Last week, I forgot to harvest and ended up harvesting...

06 December 2020 2,601 4 View