Molecular Dynamic Simulation error in GROMACS version 2020.1 (PME rank 2 are more than 2/3 times the cut-off). Is there any solution to this?

I'm running a protein-ligand Molecular Dynamic Simulation but getting the following error after hitting mdrun. Can anyone offer a solution to this? Thanks in advance. Fatal error: 66 particles...

03 February 2021 1,231 4 View

Can anyone suggest me a list of journals in computer science discipline those go for rapid publication (monthly issue actually)?

I would like to publish some articles rapidly. It would be so nice if you suggest me a list of journals those publishes monthly usually. Thanks in advance.

30 December 2020 4,648 1 View

What is the significance of H-Index and Eigenfactor in assessing the quality of a journal?

Its getting tough now a days to successfully assess the quality of a journal. H-Index and Eigenfactor are two such parameters used frequently to determine the quality of a journal. I would like...

29 December 2020 9,032 1 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

Is there any complete documentation for HTM (Hierarchical Temporal Memory)?

Currently, I am working on implementing HTM for some of state of the arts problems. I am facing some issues actually. A complete documentation may help I think.

17 September 2020 8,875 1 View

Which formula to use to calculate the sample size for a cross sectional comparative study?

If i intend to carry out a cross sectional comparative study (analytical study design) to assess the association of cardiac diseases among young stroke patients, where do I begin? Do I enroll...

07 June 2020 5,505 5 View

What is the best method of determining amplification efficiency in qPCR system?

19 April 2020 9,019 3 View

Hexokinase (HK2) Activity Assay: Can anyone suggests me, How can i measure the activity of recombinant Hexokinase 2 enzyme?

I have clone and expressed the human Hexokinase 2 gene in Pichia pastoris.Now i want to see the activities of expressed HKII protein.Can anyone provide me protocol to measures the activity of Hk2...

25 July 2019 8,757 3 View

What are the tools to design random primer?

May I know the tools or software to design the random primer for PCR? Thanks in advance.

15 January 2019 3,367 3 View

Could anyone provide carp fin tissue-genomic DNA banding pattern after gel electrophoresis?

What is the Genomic DNA banding pattern in carp fin tissue? Is it single band or smear? Could anyone provide this information? I will be grateful.

06 January 2019 4,020 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

Why cloning of gene into pCAMBIA1304 vector is unsuccessful?

I have tried to clone a specific gene into pCAMBIA1304 vector. But all the attempts failed. I used ligation kit from both Promega Corporation and Takara Bio. However, there was one time I used...

04 February 2021 525 1 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

Can someone help me identify the bands?

The samples are replicates (4) of total RNA from HEK-293 T cells. The ladder is a 1kBp ladder on a 1% native agarose gel buffered with 1x TAE. From my searches, there should be two sharp bands at...

15 January 2021 4,712 4 View

What is the possibility of PCR primers amplifying the entire vector serving as template rather than the target fragment?

My PCR primers amplify the entire vector backbone I used as a template rather than the specific amplicon. I observed this after running a gel to confirm the band's size, which ended up...

07 January 2021 7,966 3 View

What can be the reason of having high Cq-values for reference genes?

Hi, Once again I am facing an annoying problem. This time in my barley qPCR, I am trying to detect a virus (BYDV) in plant samples. I get rather high Cq-values for 2 of the 3 reference genes...

06 January 2021 4,685 9 View

Can the same primer pair , say for instance ITS 1 and ITS 4 generate different molecular weight amplicon after PCR for different fungal species?

Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?

29 December 2020 881 3 View