How to use NCBI datasets ?

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Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,227 1 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 437 3 View

How to scrape off the surface layer of compound sputtering targets as with multiple usage top layer's stoichiometry changes from the original target?

I am experiencing this for the Bi2Se3 sputtering target. Initially, even if the target was 2:3 in Bi : Se, with a few sputtering runs, due to the high escaping tendency of selenium, the top layer...

23 July 2024 1,649 1 View

What are the best ways to automate my job and live without working?

I want to know the best methods to automate my job and live without working?

12 July 2024 5,992 2 View

What are the best ways to earn more money as a researcher?

I would like know what are the best ways to earn more money as a researcher?

11 July 2024 4,693 2 View

How do I monitor my CCTV camera in Bangalore from California?

I would like to know how do I monitor my CCTV camera at my home in Bangalore from my current location which is California in USA?

11 July 2024 831 0 View

How does an AI detection tool work?

I am interested in knowing how does an AI detection tool work and what's the main logic behind it? How it is able to detect the presence of AI work?

11 July 2024 3,887 6 View

How do I check if the research work is human made or AI generated?

I would like to know how to check if a given research work is human made or AI generated?

11 July 2024 8,645 5 View

How do I prove that my research work is noval and not plagiarized?

I would like to know how to prove my research work is new and not copied from other people's work or AI tool?

09 July 2024 8,234 2 View

Is being an Assistant Professor in an university a stressful job?

I would like to know whether being an Assistant Professor in an university a highly stressful job or not?

09 July 2024 1,971 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,621 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,724 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,483 1 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,747 4 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,991 3 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,111 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Abhijeet Singh

Please read the error and modify your search.

Paul Banse

The error is

Error: NCBI Datasets provides access to assembled genome data by BioProject accession and no genome assemblies were found for the given BioProject.

Retrieval of SRA data by BioProject accession is not supported by NCBI Datasets.

Use datasets download genome taxon --help for detailed help about a command.