I was performing quenching studies in solution state. While plotting the Stern-Volmer graph, which intensity should I use as initial intensity (I0).
For example, I take 1 ml of 0.1 M fluorophore solution and add 1 ml of analyte solution in different concentrations. The resulting concentration of fluorophore in the mixed solution will be 0.05 M.
Should I consider 0.1 M or 0.05 M as the initial solution?
Hi Anjana,
Your fluorophore concentration is 0.05 M for the experiment, it doesn't matter what it was prior to mixing. You could have used 0.5 mL of 0.2 M fluorophore and 1.5 mL of analyte, and would still consider the fluorophore concentration to be 0.05 M.
Just keep in mind that the SV plot uses the concentration of quencher on its x-axis. I wasn't sure if you were using the 0.05 M fluorophore concentration for it.