Can someone provide a protocol for GSIS in Min-6 cells?

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MGA Analysis for more than two groups ?

I did MICOM and obtained partial invariance for both my demographics: teaching experience. I split the teaching experience into three groups to do Multigroup analysis However, I am not able to...

28 June 2024 3,721 4 View

Can you recommend Scopus journals, management/ business related, that provide fast acceptance?

02 May 2024 1,927 3 View

How do you sterilize olive oil to gavage to germ free mice?

Hello, I am looking for a protocol to sterilize olive oil that will then be suitable to gavage to germ free mice. Importantly, if it were to be dry-heated, could it pose a fire risk?

28 March 2024 3,760 1 View

I am measuring cell viability of OD-21 cells and my hydrogels through Alamar Blue. When i calculate to get the Blank, i sometimes get a negative?

Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?

27 March 2024 9,419 2 View

To drop or keep the item during EFA?

In my EFA, an item has high communality but loads highly on different factor. Can I still keep the item as its explained in its theory?

16 March 2024 9,170 0 View

Does anyone did Nf kB and Phospho NfkB staining flow cytometry?

I'm trying to stain NFKB and Phospho NFKB and I'm not getting any signals at all. I use True-Nuclear™ Transcription Factor Buffer Set. Does anyone have experience with staining this two ? IKB...

27 November 2023 5,822 1 View

CFA in SEM?

In my studies I have 7 IV as factors that influences 1 DV. Therefore I have 7 adapted scales and one newly established scale ( for DV) They were then translated to different language. Can I run...

25 November 2023 359 2 View

What is the best way to extract and purify a specific protien?

I am use to working with cell cultures, but I have a strong feeling that my graduate research will require me to work with different genes and proteins in specific cancer cells. So since I am a...

20 October 2023 5,079 0 View

MN9D Cells are not showing any Dopamine for HPLC analysis. Any suggestions?

We are currently working with MN9D cell line. MN9D cells are mouse midbrain that are Dopaminergic. We do several HPLC analysis on the MN9D cells for over 20 years and have always seen dopa and...

27 September 2023 750 2 View

Is K 562 cell line suitable model for BTKi studies?

Dear colleagues, I would like to assess in vitro the cytotoxic effect and possible Bruton kinase inhibition of a serries of organic compounds as potential therapeutics for the treatment of chronic...

26 July 2023 7,534 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View