Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

How can I find the best subject between architecture, economy, historical site ?

Hi there, I consider to write my final thesis in master architecture. I am trying to find a good link between economy impact , historical site and high rise buildings which I am interested in, but...

16 November 2020 4,682 16 View

Could you assist us by filling out this short survey for online instructors?

Dear colleagues, If you have shifted from teaching face-to-face to teach online, due to the COVID-19 pandemic, we would appreciate your cooperation in filling-up this short...

12 May 2020 8,532 48 View

Resource to understand silicon lithiation mechanism in lithium ion battereis?

Hi everyone, I'm looking for an article or book which explains the silicon-lithium alloy formation, which includes an account of volume change as well as the two-phase mechanism.

30 March 2020 5,880 2 View

Agarose amount in serum protein electrophoresis?

I have Mini gel tank from life technologies, I want to use it for my serum electrophoresis. Does anyone have idea about how many percentage of ready to use agarose need to order for the SPE?

26 February 2020 8,793 3 View

Do you think theoretical thematic analysis can fit with a critical realist ontology constructivist epistemology?

15 February 2020 1,397 6 View

V-REP & Python for trajectories?

I plan to use V-REP together with python for the development of trajectories in 3D CAD models, the path I will trace on the CAD file and generate a DXF file of it, Python would process it to enter...

06 November 2019 5,699 2 View

Can someone help me with instructions to mount 3D CAD files in ROS MoveIt?

I am new to using ROS MoveIt, I want to know how to load 3D CAD files of industrial parts to perform the actuator trajectory based on the cad file.

16 October 2019 5,351 2 View

does any one now of a protocol i can use for establishing a new cell line?

need to establish an homogeneous cell-line from a biopsy (cancer or polyp) thank you!! :)

13 June 2019 2,309 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View