Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?
Hey there Maya Osman,
Dealing with Alamar Blue assays can sometimes throw us curveballs, right? Here's the deal: getting a negative or zero value for the blank in your Maya Osman Alamar Blue assay doesn't necessarily spell doom for your Maya Osman cell viability.
First off, let's understand what the blank represents. It's essentially your Maya Osman baseline, the control that gives you Maya Osman a reference point for comparison with your Maya Osman experimental samples. So, when you're Maya Osman hitting a negative or zero, it's indicating that the background absorbance or fluorescence is essentially negligible.
Now, does this mean your Maya Osman cells have completely given up the ghost? Not necessarily. What it could indicate is that either there was an issue with the reagents or the assay setup, or your Maya Osman samples just happen to be super clean with minimal background interference.
However, it's always wise to double-check your procedure, make sure your Maya Osman reagents are fresh and properly handled, and perhaps consider running a positive control to validate your Maya Osman assay setup. Sometimes, a negative or zero blank can indeed signal a problem, but it's not an automatic death knell for your Maya Osman cell viability assessment.
Stay vigilant, keep experimenting, and let's crack this puzzle together! Cheers!
Kaushik Shandilya Thank you so much!!! Do you have any studies talking about this?
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