Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

How can I find the best subject between architecture, economy, historical site ?

Hi there, I consider to write my final thesis in master architecture. I am trying to find a good link between economy impact , historical site and high rise buildings which I am interested in, but...

16 November 2020 4,682 16 View

Could you assist us by filling out this short survey for online instructors?

Dear colleagues, If you have shifted from teaching face-to-face to teach online, due to the COVID-19 pandemic, we would appreciate your cooperation in filling-up this short...

12 May 2020 8,532 48 View

Resource to understand silicon lithiation mechanism in lithium ion battereis?

Hi everyone, I'm looking for an article or book which explains the silicon-lithium alloy formation, which includes an account of volume change as well as the two-phase mechanism.

30 March 2020 5,880 2 View

Agarose amount in serum protein electrophoresis?

I have Mini gel tank from life technologies, I want to use it for my serum electrophoresis. Does anyone have idea about how many percentage of ready to use agarose need to order for the SPE?

26 February 2020 8,793 3 View

Do you think theoretical thematic analysis can fit with a critical realist ontology constructivist epistemology?

15 February 2020 1,397 6 View

V-REP & Python for trajectories?

I plan to use V-REP together with python for the development of trajectories in 3D CAD models, the path I will trace on the CAD file and generate a DXF file of it, Python would process it to enter...

06 November 2019 5,699 2 View

Can someone help me with instructions to mount 3D CAD files in ROS MoveIt?

I am new to using ROS MoveIt, I want to know how to load 3D CAD files of industrial parts to perform the actuator trajectory based on the cad file.

16 October 2019 5,351 2 View

does any one now of a protocol i can use for establishing a new cell line?

need to establish an homogeneous cell-line from a biopsy (cancer or polyp) thank you!! :)

13 June 2019 2,309 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View