How do you sterilize olive oil to gavage to germ free mice?

29 March 2024 1 4K Report

Hello, I am looking for a protocol to sterilize olive oil that will then be suitable to gavage to germ free mice. Importantly, if it were to be dry-heated, could it pose a fire risk?

Robert Adolf Brinzer

Protocols say autoclave for 2h at 150-250°C. In the worst case you could probably also filter sterilize it.

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